RBS

Part:BBa_K4632023:Design

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)


BBa_B0033 gain from literature


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add 20bps random sequence before the sequence.


Source

Come from the Supplementary figures and tables including promoter sequences and plasmids used for all experiments in Jiang W, He X, Luo Y et al. Two Completely Orthogonal Quorum Sensing Systems with Self-Produced Autoinducers Enable Automatic Delayed Cascade Control[J]. ACS SYNTH BIOL, 2020,9(9):2588-2599.

References